IgMs can usually be sequenced like normal IgGs. Slightly more sample may be required. As the constant region is modified by several glycans, which cannot be cleaved by PNGase F, we can only guarantee complete coverage for the variable part of the antibody.
Our method works with all antibody formats from all species (mouse, rat, hamster, llama, rabbit, etc.; Fab, scFv, etc.).
Some mAbs are coupled to matrices. This is compatible with our sequencing method, but more samples may be required.
Many older hybridomas may produce 2 light chains (one is the kappa-chain of myeloma MOPC 21). These mixtures can usually be sequenced, even when the MOPC 21 kappa chain is in two-fold molar excess.
We also provide DASS-Based Protein and Enzyme Sequencing service including full end to end sequencing, determination of post-translational modifications, intact mass, N-glycosylation sites, and artificial tags, etc.